Protein Labeling Reagents
Biotium CF555 Tyramide
CF dye tyramide conjugates can be used for tyramide signal amplification (TSA), which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Orange-red fluorescent CF555 dye has excitation/emission at 555/565 nm.
Biotium 5-TAMRA-PEO3-amine
5-TAMRA-PEO3-amine (Tetramethylrhodamine-PEO3-amine) has a long water-soluble PEG spacer separating the dye and the amine functional group. The dye can be coupled to activated carboxy groups or sulfonyl chlorides. Properties: Ex/Em (MeOH) = 544/571 nm; Dark purple solid soluble in DMSO, DMF, or H2O; Store at 4°C and protect from light; C 37 H 42 F 6 N 4 O 11; MW: 832.74
Biotium CF594 Hydrazide
Our CF dye hydrazides are bright, extremely water-soluble and nontoxic. These properties make the dyes excellent fixable polar tracers for microinjection into cells. Hydrazides also can be used for labeling aldehyde or ketone groups, however, for this application we recommend using the more reactive CF dye aminooxy forms. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Deep-red fluorescent CF594 dye has excitation/emission at 593/614 nm.
Biotium Human Transferrin, CF488A Conjugate, 1 mg
Transferrin is a glycoprotein that binds iron for delivery to vertebrate cells through receptor-mediated endocytosis. After binding to its receptor on the cell surface, transferrin is rapidly internalized by invagination of clathrin-coated pits with formation of endocytic vesicles, the acidic environment favors dissociation of iron from the transferrin-receptor complex. Following the release of iron, the apotransferrin is recycled to the plasma membrane where it is released from its receptor to scavenge more iron. Fluorescent conjugates of human transferrin can be used as tracers for investigation of endocytosis by microscopy. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF488A dye has excitation/emission at 490/515 nm.
Biotium CF405L Protein Labeling Kit , 3x(1mg) labelings
This kit contains everything you need to label antibodies or other proteins with a fluorescent CF dye succinimidyl ester (CF dye SE). Each kit contains reagents for three labeling reactions of up to 1 mg protein per reaction. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. 405 nm-excitable green fluorescent CF405L dye has excitation/emission at 395/545 nm.
Biotium Biotin Picolyl Azide
Biotin picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency.
Biotium CF568 Tyramide
CF dye tyramide conjugates can be used for tyramide signal amplification (TSA), which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Red fluorescent CF568 dye has excitation/emission at 562/583 nm.Also see Biotium's CF Dye Tyramide Amplification Kits.
Biotium CF647 Tyramide
CF dye tyramide conjugates can be used for tyramide signal amplification (TSA), which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF647 dye has excitation/emission at 650/665 nm.
Apexbio Technology LLC Cy5 NHS ester (non-sulfonated), 10mM (in 1mL DMSO)
Cyanine5 NHS ester is a ideal reactive dye for the labeling of peptides, proteins, and oligonucleotides which contain the amino-groups. Other sizes are available. Please inqury us for quote.
Biotium DI-4-ANEPPS
Di-4-ANEPPS is a fast-responding membrane potential dye. Changes in the membrane potential of the cell correlate with changes in the fluorescence excitation intensity of the dye. Properties: Ex/ Em(MeOH) = 496/705 nm; Orange red solid soluble in DMF, ethanol or DMSO; Store at 4°C and protect from light, especially in solution; C28H36N2O3S; MW: 48; [90134-00-2]
Biotium CF488A-Dextran 10, 000 MW, Anionic and Fixable
CF dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran conjugates are available in a variety of molecular weights, and contain a formaldehyde-fixable free-amine group. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF488A dye has excitation/emission at 490/515 nm.
Biotium Tyramide Amplification Kit with HRP Streptavidin and Biotin-XX Tyramide
Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to the conventional avidin-biotinylated complex procedure. The kit contains HRP streptavidin Biotin-XX tyramide. The kit contains sufficient reagent for 50-150 slides.
Biotium GloMelt™ Thermal Shift Protein Stability Kit
GloMelt™ dye undergoes fluorescence enhancement upon binding to hydrophobic regions of denatured proteins. Therefore the dye can be used to detect protein unfolding or measure thermal stability by performing a thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay. GloMelt™ dye has significant advantages over other environmentally sensitive dyes, such as SYPRO Orange and PROTEOSTAT TS dye. GloMelt™ dye generates a strong signal because it is optimized for detection in the SYBR Green channel of qPCR instruments, and therefore low reaction volumes and low protein concentrations can be used. GloMelt™ dye is compatible with high concentrations of protein stabilizers (such as glycerol and sorbitol), and also protein destabilizers. GloMelt™ dye performs very well in high detergent concentrations, unlike SYPRO Orange. This kit contains no ROX reference dye and has sufficient reagent for 2000 assays.
Sigma Aldrich Fine Chemicals Biosciences Phalloidin fluorescein Iso1MG
Phalloidin is a phallotoxin produced by death cap mushroom Amanita phalloides. It is a cyclic peptide which interacts with actin and this was first identified in phalloidin-poisoned rats. It is a heptapeptide cyclic in nature with a crosslink between tryptophan at position 6 and cysteine at position 3. The side chain of amino acid 7 (I3-δ-dihydroxyleucine) in phalloidin is accessible to modifications through which fluorescently labelled phalloidin compounds can be produced.
Sigma Aldrich Fine Chemicals Biosciences Sera MagTM Magnetic carbox
Sera-Mag(TM) SpeedBeads(TM) and Sera-Mag(TM) Carboxylate-Modified Magnetic Particles combine a fast magnetic response time and high binding capacity with a large surface area high sensitivity stability physical integrity and fast reaction kinetics. Covalent coupling of biomolecules such as proteins and nucleic acids to carboxyl groups on the surface is easily accomplished using our standard coupling technology. The core of the particle is made by a free radical emulsion polymerization of styrene and acid monomer. One (Sera-Mag(TM)) or two (Sera-Mag(TM) SpeedBeads(TM)) layers of magnetite are then coated onto this core while the surface is modified to minimize non-specific binding of proteins.